The Cristalino Jungle Lodge is nestled in a private forest reserve in the Southern Brazilian Amazon rainforest. It borders the Cristalino State Park and is therefore part of a continuous extensive protected area that extends northward into the adjacent state of Pará. This unique region contains many species found only in this part of the Amazon. The area contains pristine forest and aquatic habitats that support a very rich fauna and flora, and it has been described as an Amazon sanctuary.

The lodge has become an internationally famous destination for nature lovers of all types, in particular birders and butterfly enthusiasts, but also attracts those seeking adventure in a peaceful, exotic location. Facilities include an extensive trail system, a new 50 meter tall tower that rises above the forest canopy, and a clean black-water river where one can canoe and swim. Well-trained resident guides assist visitors to find interesting wildlife and to interpret what they see while simultaneously looking after their safety. Cristalino has equipment for use in the forest and a library to help visitors identify and learn about what they see, and the lodge is designed to combine rustic comfort with respect for nature. The number of tourists that can visit Cristalino at any one time is limited to ensure that there is minimal environmental impact.

Tourism is not the only function of Cristalino. Their most important objective is to preserve the rainforest and to contribute to conservation efforts in part of the Amazon that is rapidly changing. With this in mind, Cristalino encourages and supports certain scientific research projects in the area, and their Escola da Amazônia holds regular workshops at the hotel and forest fragment in Alta Floresta. These workshops cater mainly for high school and university students and aim to increase their awareness of environmental issues and respect for nature.